... [green, blue, red] V) Light source [fluorescent] VI) Sodium bicarbonate [NaHCO3] 0.2% solution VII) Broad bean seedlings. In a nutshell, by the use of vacuum, produced O2 [by photosynthesis] is sucked out of the leaf discs [which were cut from a young broad bean seedling] with no air in the leaves the disc start to sink to the bottom in either a dH2O or a NaHCO3 solution, then a fluorescent light is placed [different or no filters for different groups] so that the discs can once again start photosynthesis. If the photosynthetic reaction is a success O2 will be produced again and the disc will once again surface. This experiment involves a few variables, one variable which will be varied are the quality of light, chlorophyll best absorbs light in the wavelength range of 400-450 nm [blue light] and 650-700 nm [red light]. Chlorophyll does not absorb green light or yellow light ...

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