... 'running' gel. The stacking gel is at pH 6.9, while at this pH, glycine exists as zwitterions so it has no net charge. After PAGE, Rf values of all proteins are calculated, which is a measure of relative mobility: Relative Mobility (Rf) = Protein composition of mixture X is determined from the plot of log (molecular weight) against Rf. Procedure Please refer to the laboratory manual. Precaution 1. Hand glove must be worn to prevent skin contact with monomer acrylamide, which is neurotoxic. 2. All powder form of neurotoxic acrylamide should be dealt with care as it can easily be inhaled. 3. For electrical insulation, the chamber should be sealed along its edges with Vaseline and sticky tape 4. Light sensitive ammonium persulphate should be covered with aluminium foil. 5. Sample solvent should be freshly prepared, as under prolonged exposure to oxygen in air, the reducing power of ß-mercaptoethanol decreases with time. 6. Staining and destaining solution should be ...

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