... this it is used worldwide in molecular biology. There are many applications of PCR, all of which have aided the current knowledge into DNA sequencing and cloning. PCR is frequently used when screening gene libraries after standard cloning experiments. This method can identify and isolate a clone carrying a particular region of the genome from the thousands of similar clones that contribute a gene bank. In this case PCR is known as 'in vitro amplification' as it utilises two oligonucleotide primers that flank the regions of interest. Once these cloned fragments have been amplified, they can be easily screened to allow many copies to be available for research purposes. PCR has established an important role in forensic analysis, enabling genetic fingerprinting techniques to be used to test and assay small amounts of DNA, found in single hair, bloodstains and bones. Genetic profiling is used to identify differences in the human genome in ...

See this essay or coursework document in full right now