... acids straightened out. In denaturing, (SDS PAGE) separations, migration is determined not by intrinsic electrical charge of the polypeptide but by molecular weight between log molecular weight and (RF) distance migrated by the protein/ distance migrated by the dye front. It is usually necessary to reduce disulphide bonds in proteins before they adopt the random coil configuration necessary for separation by size; this is achieved with the addition of 2-mercaptoethanol or dithiothreitol which was carried out in this investigation. To be able to determine the subsequent separated serum proteins, Western blot is a technique that is used in order to make the proteins accessible to antibody detection Western blot is a method to detect a certain protein in a sample by using antibody specific to that protein and the size of the protein. The separating gel is transferred onto a nitrocellulose membrane; this is the actual blotting process and is necessary ...

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