... acrylamide and bisacrylamide. When these two are combined a porous network is formed. The former determines the average polyacrylamide chain length. The latter determines the extent of the cross linking. The concentration of gel and the pore size is inversely proportional. To prepare this gel; we do not need to boil and then cool as we did to agarose. We need some assistant chemicals to prepare it. The chemicals are TEMED and APS. The preparation order is first the APS and then TEMED, to start polymerization the mixture has to be stirred. Tetramethyletyl Diamine (TEMED): This chemical is the initiator of the polymerization of polyacrylamide. Ammoniumpersulfate (APS): This is the catalyst of the reaction. Sodium dodecyl sulphate (SDS) and 2-Mercaptoethanol: SDS is an anionic detergent and mercaptoethanol is a reducing agent. SDS binds to the protein, causing it to unfold, whereas the reducing agent reduces the intramolecular and intermolecular disulfide bonds. Therefore the ...

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