... predicted. Fragmentation of ?-DNA using R.E.'s (Restriction-Enzymes) EcoR1, BamH1 and HindIII So, from this, the bands and smears that appear could be predicted. It would also be reasonable to show that the column with no restriction enzymes present would have no fragmentation of the ?-DNA, as the ?-DNA would not be broken down. For EcoRI: There will be one band near the start (21 226 base pairs fragment) as it is the biggest and so will travel the shortest in the gel electrophoresis. There will be another band about 2-3 times as far as the first, which is the 7 421 base pair fragment. I'm reasoning it will be about 2-3 times as far because it is around a third of the size. Next, there will be either one smear or several fragments depending on how far the remaining fragments have moved. As they are all similar in size then they ...

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