... controlled steps of sample preparation, 2-D electrophoresis, image detection and analysis, spot identification, and database searches" 2. The main core of the technology and advances in the study of proteomics is due to the application of 2D electrophoresis and as it stands at the moment there is no alternative method available for resolving thousands of proteins in one separation technique. An Introduction to Two Dimensional Electrophoresis. The most widely used tool in proteomics used to analyse proteins is the technique of Two-dimensional gel electrophoresis, which is often abbreviated as either 2DE or 2-D Electrophoresis. This is a two step process technique which separates proteins in accordance to two independent properties, there isoelectric point (pI) and there relative molecular weight (Mr) 2. In the first dimension the proteins or analytes will lie along a line separated by their pI, in 2DE the technique starts with 1DE then in the second dimension the ...

See this essay or coursework document in full right now