Gain Immediate access to our Essays
FREE access exchanged for your work, or pay £9.99
Words: | Submitted: Mon Sep 08 2003
... then flick the side repeatedly with a finger to mix the contents. Do this for one full minute.> Allow the tube to stand for a further 5 minutes. The Lambda DNA solution should look slightly opaque.> Add a fresh tip to the microsyringe. Put 20 ul of Lambda ?DNA solution into an enzyme tube of your choice (refer to Fig. 4 for the colour code). Mix the liquid and the dried enzyme by carefully drawing the liquid up and down in the tip a few times.> Repeat this for each enzyme tube and the yellow 'control' tube, using a fresh tip each time to prevent cross-contamination between the tubes.> Cap each tube with an appropriately coloured lid.> Incubate the tubes at 37 ?C, in a water bath or incubator, for between 30 and 45 minutes.> Place the electrophoresis tank on a level surface, where you can leave it undisturbed for ...
FREE access exchanged for your work, or pay £9.99