... 6.7 1% NaCl solution Fearons reagent 20% NaOH solution pH 6.7 buffer Amylase solution Distilled water Tongs Boiling tubes Boiling tube rack 100 cm3 measuring cylinder 1, 5 and 10 cm3 pipettes and safety pumps Gloves Goggles Cuvettes Stop clock Colorimeter Ice Bath (0 oC), Water baths Bunsen burner Tripod Gauze Heatproof Mat Safety * Lab coat (protect clothes) * Safety goggles (protective eye care) * Rubber gloves (protect hands. 20% NaOH is a corrosive irritant) Method * Firstly put all safety equipment on (lab coat, safety glasses and gloves) * Prepare 7 test tubes, label them s1 to s7. * Place 5cm3 of buffered starch, 2cm3 of pH 6.7 buffer, 1cm3 of 1% NaCl in each of them. * Using a pipette place 5cm3 of the amylase solution into 7 separate tubes (labelled E1 to E7). * Tubes S1 and E1 in to the ice bath * Tubes S2 and E2 in to the room temperature water bath * Tubes S3 and E3 in to a water bath at 30oc. * Tubes S4 and E4 in to a water bath at 40oc. * Tubes ...

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