... reaction mixture contains simply hydrogen peroxide (substrate) and yeast (as the source of enzyme) with or without copper sulphate. Adding yeast will start the reaction, and then the measurements will be taken immediately. A brief method of carrying out this experiment would be to react constant amounts of, yeast (catalase), constant amounts of the variable in concern (copper sulphate), this is part of the fair test, because varying the amount of the enzyme in both the reaction would give results without trends that follow each other, reacting with various amounts of the substrate, Hydrogen peroxide, in solutions with different concentrations i.e. varying the amount of water in the solution. The yeast would be the last substance added (because at the moment which it is added, the reaction begins) at which moment the stop clock would start ticking measuring the amount of oxygen produced in the reaction at time ranges from 0-140seconds, at ...

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