... mirabilis identification Urine samples were spread onto CLED agar plates (prevents swarming of Proteus) and blood agar plates (identifies hemolytic strains) and incubated overnight at 37oC. Characteristic Proteus colonies were identified on CLED agar (these being blue, smooth convex colonies), and a swarming colony covered the blood agar. An API test was set up according to the kit instructions and a BLAST sequencing method was also set up to determine whether the species was P. mirabilis. 2. Morphology Cell morphology was investigated by inoculating the centre of a nutrient agar (NA) plate, and some nutrient broth (NB) with a colony from the CLED plate and examining cells from the plates under the electron microscope. 3. Chemotaxis a) Chemotaxis chamber: One well and half of the channel was filled with 10% nutrient agar. The opposite well and the rest of the channel were filled with 1M hydrochloric acid. This was repeated in other chambers substituting 1M ...

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