... the exchangers: changing pH of the buffer, increasing the ionic strength and performing an affinity solution. When the pH or the ionic strength are changed, the electrostatic binding between the absorbed ion and the exchanger are weakened. The absorbed ion can be eluted. The affinity solution weaken the electrostatic interaction between the ion and the exchangers by lowering the net charge on the macromolecules, so that It can also elute the ion out. Lastly, the amount of tyrosine eluted out is determined by the absorbance. The absorbance is directly proportional with the amount of tyrosine. Result: The volume of the tyrosine used : 0.5ml Tube no. Conductivity (1000 uS) Absorbance of tyrosine at 280nm Tube no. Conductivity (1000 uS) Absorbance of tyrosine at 280nm 1 33.5 0.007 11 38.9 0.236 2 36.0 0.017 12 39.2 0.137 3 36.3 0.006 13 39.4 0.021 4 36.7 0.022 14 39.7 0.003 5 37.1 0.009 15 39.9 0.005 6 37.4 0.020 16 40.2 0.001 7 37.8 0.051 17 40.3 0.055 8 38.1 0.007 18 40.4 0.020 9 38.5 0.008 19 40.6 / 10 38.7 0.043 20 40.7 0.034 Table that show the conductivity and the relative absorbance of the tubes From the table, there is the highest absorbance at tube 11, the respective conductivity is 38.9uS. by the equation, y= 5.758x + 31.778 : where y is the ...

See this essay or coursework document in full right now