... moved by solvent Safety * Local rules apply * The sealing of melting point tubes by Bunsen must be done away from flammable solvents * After practical all waste solvents much be disposed in appropriate receptacles * Do not look directly at UV light Method Each group is given 2 unknown compounds to identify Thin Layer Chromatography 1. Prepare solution with 10ml ethanol in 90ml hexane and place in a sealed chromatography tank at least 20 minutes before analysis 2. Prepare two fine needles using Bunsen burner. 3. Take a few crystals of the unknown compound and dissolve it in 1ml of chloroform then spot the solution onto the chromatoplate using the 2 needles prepared. 4. Place the chromtoplate in the sealed tank. 5. Remove the plate once the solvent has travelled near to the top and use UV light to visualise the spots and determine the Rf value. Melting Point Determination 1. Seal one end of a melting point tube in the Bunsen burner ...

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