... amylase becomes damaged and becomes denatured Prediction From my research I predict that as the heat increases the iodine solution will turn black less because of sugar being produced and the reaction happening quicker with more heat but after a bit above body temperature the reaction will be denatured and turn the iodine black. Variables Keep the Same Change Amount of Starch (10ml) Temperature Time for recording (20sec) Amount of Amylase (5ml) Apparatus ? Bunsen Burner ? 10ml measuring cylinder ? Thermometer ? Stop clock ? Iodine solution ? Starch ? Amylase ? Spotting tile ? Pipettes ? Two test tubes ? Tongs Method ? Set up apparatus listed above. ? Place Iodine solution into the spotting tile. ? Heat Starch and Amylase separately to the right temperature allocated in results ? Mix the amylase and starch solution and start the stop clock ? Every 20 seconds add a couple of drops of amylase and starch mixture to the iodine spot. ? Record the colour in the results. ? Repeat at different temperatures ? Repeat the experiment using the same ...

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