... times whilst doing the experiment *Heat the rim of the culture bottle to prevent airborne contamination. *Flame the tongs in Bunsen burner after each go to kill all microorganisms. Apparatus List * Bunsen burner * Tongs * Agar plate * Inoculating loop Step By Step Method 1. First light the Bunsen burner 2. Adjust the Bunsen burner to a roaring blue flame 3. Then flame the inoculating loop in the roaring blue flame to ensure sure that it is free of any micro organisms from past experiments will may contaminate culture. 4. Also flame the neck of the bottle for the same reason 5. Insert loop into the bottle of yeast for 10 to 15 seconds 6. Remove loop, there should be a thin film of yeast culture in the loop 7. Lift lid of agar plate ajar (be careful when doing so to avoid airborne contamination) just high enough to fit loop inside the plate 8. Streak the plate with the yeast culture ...

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